Site Directed Mutagenesis (SDM) Human Point mutation Caco-2

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Human Seminal vesicles

Reporter gene assays are designed to test the regulation of the expression of a gene of interest. This is usually done by linking the promoter of the gene of interest with a gene such as a firefly luciferase, which can be easily detected by addition of luciferin that leads to an enzymatic reaction to produce luminescence. The enzymatic reaction can be correlated to the expression of the gene of interest. Another luciferase gene that can be used is Renilla luciferase. For an appropriate luciferase assay: 1. the reporter should express uniformly in all cells, 2. specifically respond to effectors that the assay intends to monitor, 3. have low intrinsic stability to quickly reflect transcriptional dynamics. It is important to have an equal number of cells plated in each testing condition to avoid any incorrect readouts. Reporter assays could be single or dual reporter assays. The reporter could be both luciferases. Most dual-luciferase assays involve adding two reagents to each sample and measuring luminescence following each addition. Adding the first reagent activates the first luciferase reporter reaction; adding the second reagent extinguishes first luciferase reporter activity and initiates the second luciferase reaction. Dual-luciferase assays have some advantages, including 1. reduces variability, 2. reduces background, 3. normalizes differences in transfection efficiencies between samples.

Get tips on using siRNA Wee1 to perform siRNA / miRNA gene silencing Human - SKOV-3 Wee-1

Get tips on using Hs_PTEN_6 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - HCT-116 PTEN1

Get tips on using Hs_DNMT3B_2 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - HCT-116 DNMT3B

Get tips on using Hs_IFI16_1 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - TF‐1 IFI16

Get tips on using YAP siRNA (h) to perform siRNA / miRNA gene silencing Human - OV2008 Yap Gene

Get tips on using Hs_GLI1_1 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - rh-36 GLI1

Get tips on using Hs_HDAC2_1 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - NCI-H460 HDAC2_1

Get tips on using Hs_HDAC1_6 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - NCI-H460 HDAC1_6