Get tips on using QuantiFluor® dsDNA System to perform DNA quantification Colorectal aenocarenoma (SW48) - paraffin embeded
Get tips on using Allprotect Tissue Reagent (100 ml) to perform Stabilization of DNA Cervico-vaginal smears
Get tips on using QIAamp UCP Pathogen Mini Kit to perform DNA isolation / purification Tissue - bronchoalveolar lavage
Get tips on using QuantiNova Pathogen +IC Kit (500) to perform PCR Conventional / Qualitative PCR - mammalian DNA
Get tips on using QuantiTect Probe PCR Kit (1000) to perform PCR Conventional / Qualitative PCR - bacterial DNA
Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - MDA-MB-231
Get tips on using Taq PCR Master Mix Kit to perform PCR Conventional / Qualitative PCR - mammalian DNA
Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification MCF10A breast epithelial cells
DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.
Get tips on using QIAGEN Plasmid Plus Midi Kit (25) to perform DNA isolation / purification Plasmid purification
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment