rna-isolation-purification-cells-primary-rat-brain-microvascular-endothelial-cells

Get tips on using RNeasy PowerBiofilm Kit (50) to perform RNA isolation / purification Biofilm

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using PE Rat Anti-Mouse CD103 to perform Flow cytometry Anti-bodies Mouse - CD103

Get tips on using PE Rat Anti-Mouse CD25 to perform Flow cytometry Anti-bodies Mouse - CD25

Get tips on using PE Rat Anti-Mouse CD49b to perform Flow cytometry Anti-bodies Mouse - CD49b

Get tips on using PE Rat Anti-Mouse CD73 to perform Flow cytometry Anti-bodies Mouse - CD73

Get tips on using PerCP Rat Anti-Mouse CD8a to perform Flow cytometry Anti-bodies Mouse - CD8a

Get tips on using FITC Rat Anti-Mouse CD4 to perform Flow cytometry Anti-bodies Mouse - CD4

Get tips on using FITC Rat Anti-Mouse CD74 to perform Flow cytometry Anti-bodies Mouse - CD74

Get tips on using PE Rat anti-Mouse CD34 to perform Flow cytometry Anti-bodies Mouse - CD34