shRNA gene silencing Human THP-1

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using GeneArt™ Site-Directed Mutagenesis PLUS System to perform Site Directed Mutagenesis (SDM) Human - Insertion SKOV3 miR-134

Get tips on using 1kb DNA Ladder to perform DNA Ladder 1 kb

Get tips on using 1kb DNA Step Ladder to perform DNA Ladder 1 kb

Get tips on using BenchTop 1kb DNA Ladder to perform DNA Ladder 1 kb

Get tips on using GeneArt™ Site-Directed Mutagenesis System to perform Site Directed Mutagenesis (SDM) Mouse - 3T3-L1 S6 kinase 1

Get tips on using DNA Ladder & Loading Dye to perform DNA Ladder 1 kb

Get tips on using Annexin V-FITC Apoptosis Kit to perform Necrosis PANC-1

Get tips on using LC3B Antibody #2775 to perform Autophagy assay cell type - TPC 1