siRNA / miRNA gene silencing Human 501 Mel and SK Mel 28 FANCD2

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using Human Von Willebrand Factor ELISA Kit (VWF) (ab108918) to perform ELISA Human - VWF-A2

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Get tips on using Human KIM1 / TIM-1 PicoKine™ ELISA Kit to perform ELISA Human - KIM-1

Get tips on using Human TIM-1/KIM-1/HAVCR DuoSet ELISA to perform ELISA Human - KIM-1

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Get tips on using Human Total HO-1/HMOX1 DuoSet IC ELISA to perform ELISA Human - HO-1

Get tips on using Tau Protein Ladder, 6 isoforms human to perform Protein Ladder Immunofluorescence

Get tips on using RosetteSep™ Human Monocyte Enrichment Cocktail to perform Cell Isolation Monocyte