siRNA / miRNA gene silencing Human NCL-H40

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Repression

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Human - Glioblastoma stem-like cells (GSCs)

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Thyroid gland

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Spinal cord

Get tips on using BioPrime™ Array CGH Genomic Labeling Module to perform Microarray Comperative genomic hybridization - Human PBMCs

Get tips on using NucleoSpin® RNA XS to perform RNA isolation / purification Tissue - Human Skin

Get tips on using NucleoSpin® RNA/Protein to perform RNA isolation / purification Tissue - Human Cornea

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using Gentra Puregene Cell Kit to perform DNA isolation / purification Cells - Primary cells Human primary keratinocytes

Get tips on using PyroMark CpG Assays to perform DNA methylation profiling Whole genome profiling - human whole blood