RNA isolation / purification Plants

Get tips on using QIAfilter Plasmid Kits to perform Plasmid Isolation E. coli clinical isolate

Get tips on using HiSpeed Plasmid Midi Kit (25) to perform Plasmid Isolation E. coli

Get tips on using E.Z.N.A.® Plasmid Midi Kit to perform Plasmid Isolation Yersinia enterocolitica

Get tips on using TIANpure Mini Plasmid Kit(DP104) to perform Plasmid Isolation Citrobacter freundii

Get tips on using PureYield™ Plasmid Midiprep System to perform Plasmid Isolation Citrobacter freundii

Get tips on using PureYield™ Plasmid Midiprep System to perform Plasmid Isolation Streptococcus pneumoniae

Get tips on using Plasmid Miniprep Kit I, peqGOLD to perform Plasmid Isolation Staphylococcus aureus

Get tips on using GenElute™ Plasmid Miniprep Kit to perform Plasmid Isolation S. cerevisiae

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.