siRNA / miRNA gene silencing Human Primary Human Hepatocytes

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using MitoSOX™ Red Mitochondrial Superoxide Indicator, for live-cell imaging to perform ROS assay cell type - A549 human adenocarcinomic human alveolar basal epithelial cells

Get tips on using miRNeasy Serum/Plasma Kit to perform RNA isolation / purification Tissue - Human Blood / Serum / Plasma / Buffy coat

Get tips on using pLX-sgRNA to perform CRISPR Human - Deletion CIITA

Get tips on using CDX2 to perform Immunohistochemistry Human - CDX2

Get tips on using Cas9 sgRNA vector to perform CRISPR Human - Activation ESR1

Get tips on using PCR Sequencing Kit to perform Whole Genome Amplification Human

Get tips on using Rapid Sequencing Kit to perform Whole Genome Amplification Human

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Human - H9