I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?
Get tips on using Wizard® Plus Maxipreps DNA Purification System to perform Plasmid Isolation E. coli DH5α
Get tips on using Illustra GFX PCR DNA and Gel Band Purification kit to perform DNA gel extraction / PCR product purification Product size < 15Kb
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