DNA transfection Mammalian cells Primary cells

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DNA DNA isolation / purification Tissue small intestine

DNA DNA isolation / purification Micorbiome Fecal sample

DNA DNA isolation / purification Micorbiome Human skin

DNA DNA isolation / purification Micorbiome Human gut

DNA DNA isolation / purification Tissue bronchoalveolar lavage

DNA DNA isolation / purification Plants Mycelial mats

DNA PCR Conventional / Qualitative PCR Viral DNA

I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

Discussions DNA insert using CRISPR

Get tips on using Wizard® Plus Maxipreps DNA Purification System to perform Plasmid Isolation E. coli DH5α

Products Promega Wizard® Plus Maxipreps DNA Purification System

Get tips on using Illustra GFX PCR DNA and Gel Band Purification kit to perform DNA gel extraction / PCR product purification Product size < 15Kb

Products Fisher Scientific Illustra GFX PCR DNA and Gel Band Purification kit

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