siRNA / miRNA gene silencing Human NCL-H40

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion SLX4

Get tips on using phREX1-Luc to perform CRISPR Human - Activation REX1

Get tips on using SOX9 Polyclonal Antibody to perform Immunohistochemistry Human - SOX9

Get tips on using Dicer Antibody (CL0378) to perform Immunohistochemistry Human - Dicer1

Get tips on using MSH2 Polyclonal antibody to perform Immunohistochemistry Human - MSH2

Get tips on using CRISP3 Polyclonal antibody to perform Immunohistochemistry Human - CRISP3

Get tips on using Villin Monoclonal antibody to perform Immunohistochemistry Human - Villin

Get tips on using EZ-ChIP™ to perform ChIP Human - HUVEC

Get tips on using MethylCap kit to perform DNA methylation profiling Whole genome profiling - DU145, PC3 human prostate cancer

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.