dna-quantification-human-pc-3

Get tips on using Amino Allyl MessageAmp™ II aRNA Amplification Kit to perform RNA amplification & labeling Fish - Total RNA, Fundulus heteroclitus Cyanine 3 & 5

Get tips on using APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU to perform Apoptosis assay cell type - BxPC-3

Get tips on using DNeasy UltraClean 96 Microbial Kit (384) to perform DNA isolation / purification Bacteria - Gram negative E.coli

Get tips on using DNeasy PowerSoil HTP 96 Kit (384) to perform DNA isolation / purification Bacteria - Gram negative E.coli

Get tips on using DNeasy PowerSoil HTP 96 Kit (384) to perform DNA isolation / purification Bacteria - Gram positive Pseudomonas

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using dam Methyltransferase to perform PCR Methylation specific PCR - Bacterial DNA

Get tips on using PCR SuperMix to perform PCR Conventional / Qualitative PCR - mammalian DNA

Get tips on using Sodium bisulfite to perform PCR Methylation specific PCR - Bacterial DNA

Get tips on using HaeIII Methyltransferase to perform PCR Methylation specific PCR - Bacterial DNA