shRNA gene silencing Human HEK 293T CAPN5- (Calpains)

Get tips on using Phusion Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Human - Deletion HepG2 Keratin 14

Get tips on using DeadEnd™ Fluorometric TUNEL System to perform TUNEL assay cell type - HeLa cells human cervical cancer

Microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using Corning® Hepatocyte Culture Media Kit, 500 mL to perform 3D Cell Culture Media Human bladder cancer organoid

Get tips on using MethylCap kit to perform DNA methylation profiling Whole genome profiling - DU145, PC3 human prostate cancer

Get tips on using Unrestricted HD-CGH Microarray ISCA v2, 4x44k to perform Microarray Comperative genomic hybridization - Human PBMCs

I work with Human liver organoids and I would like to know the impact of FBS on the organoids. Since the composition of FBS is unknown, would you recommend any alternatives such as human platelet lysate?

Get tips on using STEMdiff™ Definitive Endoderm Kit to perform Stem cell Differentiation media Differentiation of Human hESCs into Endoderm

Get tips on using Oris™ Pro Cell Migration Assay to perform Wound healing assay cell type - human MDA-MB-231

Get tips on using Q5® Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Human - Point mutation HepG2 SMAD3