Get tips on using Mouse VEGF PicoKine™ Fast ELISA Kit to perform ELISA Mouse - VEGF
Get tips on using Mouse Total MMP-9 Quantikine ELISA Kit to perform ELISA Mouse - MMP9
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
Get tips on using Human/Mouse NLRP3/NALP3 Antibody to perform Western blotting NLRP3
Get tips on using PCNA (PC10) Mouse mAb #2586 to perform Western blotting PCNA
Get tips on using Mouse GDNF ELISA Kit (ab171178) to perform ELISA Human - GDNF
Get tips on using Human/Mouse BDNF DuoSet ELISA to perform ELISA Human - BDNF
Get tips on using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® to perform RNA sequencing Mouse - Neuro 2a
Get tips on using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® to perform RNA sequencing Mouse - BV-2
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