ChIP acH4 Bovine Human

Get tips on using Anti-CTCF Antibody to perform ChIP Anti-bodies CTCF

Get tips on using Anti-Ctip1/BCL-11A antibody [14B5] (ab19487) to perform ChIP Anti-bodies CtIP/BCL11A

Get tips on using Histone H2A.X antibody (pAb) to perform ChIP Anti-bodies γH2AX

Get tips on using Histone H4K20me1 antibody (mAb) to perform ChIP Anti-bodies H4K20me1

Get tips on using Histone H3K36me3 antibody (mAb) to perform ChIP Anti-bodies H3K36me3

Get tips on using Histone H3K9me3 antibody (mAb) to perform ChIP Anti-bodies H3K9me3

Get tips on using Histone H3K4me3 antibody (mAb) to perform ChIP Anti-bodies H3K27me1

Get tips on using Histone H3K27ac antibody (pAb) to perform ChIP Anti-bodies H3K27ac

Get tips on using Histone H3K4me3 antibody (mAb) to perform ChIP Anti-bodies H3K4me3

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.