siRNA / RNAi /miRNA transfection Rat IEC-6

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using Anti-Calretinin Antibody, clone 6B8.2 to perform Immunohistochemistry Mouse - Calb2

Get tips on using Fibronectin Antibody (617): sc-81769 to perform Western blotting Fibronectin

Get tips on using Vimentin Antibody (5G3F10): sc-66002 to perform Western blotting Vimentin

Get tips on using GAPDH Antibody (6C5): sc-32233 to perform Western blotting GAPDH

Get tips on using P-cadherin Monoclonal Antibody (6A9) to perform Western blotting P-Cadherin

Get tips on using IGEPAL® CA-630 to perform Protein isolation Mammalian cells - Human lung fibroblasts

Get tips on using CD24 Antibody (M1/69): sc-19651 to perform Immunohistochemistry Mouse - CD24

Get tips on using p21 Antibody (F-5): sc-6246 to perform Western blotting p21

Get tips on using miScript PreAMP PCR Kit (60) to perform PCR Preamplification of cDNA - FFPE samples