Site Directed Mutagenesis (SDM) Human Point mutation PC-3

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion TLN2

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion SLX4

Get tips on using phREX1-Luc to perform CRISPR Human - Activation REX1

Get tips on using SOX9 Polyclonal Antibody to perform Immunohistochemistry Human - SOX9

Get tips on using Dicer Antibody (CL0378) to perform Immunohistochemistry Human - Dicer1

Get tips on using MSH2 Polyclonal antibody to perform Immunohistochemistry Human - MSH2

Get tips on using CRISP3 Polyclonal antibody to perform Immunohistochemistry Human - CRISP3

Get tips on using Villin Monoclonal antibody to perform Immunohistochemistry Human - Villin

Get tips on using EZ-ChIP™ to perform ChIP Human - HUVEC

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.