DNA quantification Human

Get tips on using CDX2 to perform Immunohistochemistry Human - CDX2

Get tips on using APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU to perform DNA Damage Assay U-2 OS

Get tips on using FuGENE® 6 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat aortic smooth muscle cells (rASMC)

Get tips on using Gentra Puregene Cell Kit Plus (6.7 x 109) to perform DNA isolation / purification Cells - Immortalized cell lines H1 hESc

Get tips on using EZ-10 Spin Column PCR Product Purification kit to perform DNA gel extraction / PCR product purification Product size > 15Kb

Get tips on using s4215 to perform siRNA / miRNA gene silencing Human - PANC-1 DNMT1/3b

Get tips on using TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG to perform PCR Multiplex PCR - Mammalian DNA

Get tips on using pcDNA-dCas9-p300 Core to perform CRISPR Human - Activation MASPIN

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using MLM3636 to perform CRISPR Human - Activation VEGFA