mRNA / Ribonucleoprotein isolation / purification

Get tips on using exoRNeasy Midi Kit (50) to perform Purification of extracellular vesicles Exosomes - Plasma

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

Get tips on using Monarch® Plasmid Miniprep Kit to perform Plasmid Isolation E. coli INVαF'

Get tips on using IMPACT™ Kit to perform Protein expression and purification Bacteria - Escherichia coli GmrSD

Get tips on using pGS-21a Vector to perform Protein expression and purification Bacteria - Escherichia coli p19

Get tips on using Ni-NTA Agarose to perform Protein expression and purification Insect cells - Hi5 TYR

Get tips on using QIAexpress Type IV Kit to perform Protein tag Purification of His-tagged proteins

Get tips on using GeneRead Size Selection Kit (50) to perform Whole Genome Amplification NGS library purification

Get tips on using pET-21a(+) DNA to perform Protein expression and purification Bacteria - Escherichia coli Prefoldin (PFD)

Get tips on using pQE-80L vector to perform Protein expression and purification Bacteria - Escherichia coli IFABP-Aβ