DNA transfection Mammalian cells Immortalized cell lines

Get tips on using T-PER™ Tissue Protein Extraction Reagent to perform Protein isolation Mammalian cells - Huh7

Get tips on using Pierce™ Coomassie (Bradford) Protein Assay Kit to perform Protein quantification Mammalian cells - RAW264.7

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay MIA PaCa-2

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay MDA-MB-231

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay U-87 MG

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay U-2 OS

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using Quick-Load® Purple Low Molecular Weight DNA Ladder to perform DNA Ladder Low Range

Get tips on using BH100bp ladder :: GD 100bp DNA Ladder H3 RTU Ladder to perform DNA Ladder 100 bp

Get tips on using Quick-Load® Purple 1 kb Plus DNA Ladder to perform DNA Ladder 1 kb