Site Directed Mutagenesis (SDM) Human Deletion MDA-MB-231

Get tips on using OCT4-PG1 siRNA to perform siRNA / miRNA gene silencing Human - hES cell line H1 (WA01) OCT4-PG1

Get tips on using connexin 43 ShRNA to perform shRNA gene silencing Human - Neuroblastoma cells (SH-SY5Y) Connexin 43 lentiviral particles

Get tips on using 14-3-3ζ siRNA(h) to perform siRNA / miRNA gene silencing Human - Caco-2 14‐3‐3ζ

Get tips on using Rock-2 siRNA and shRNA Plasmids (h) to perform siRNA / miRNA gene silencing Human - HT-1376 ROCK2

Get tips on using SuperFect Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human aortic smooth muscle cells (HOSMC)

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human pulmonary arterial smooth muscle cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human umbilical vein smooth muscle cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human umbilical artery smooth muscle cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human pulmonary artery smooth muscle cells