Site Directed Mutagenesis (SDM) Rat Point mutation

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Cell cytotoxicity / Proliferation assay cell type - K562

Get tips on using LC3B (D11) XP® Rabbit mAb to perform Autophagy assay cell type - HeLa

Get tips on using Anti-LC3 antibody produced in rabbit to perform Autophagy assay cell type - B16F10

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - H4

Get tips on using Anti-ATG4B antibody produced in rabbit to perform Autophagy assay cell type - H4

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using CD34 Monoclonal Antibody (RAM34), eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD34

Get tips on using ICAM-1 Recombinant Rabbit Monoclonal Antibody (9H21L19) to perform Western blotting ICAM-1

Get tips on using Cyclin D1 Recombinant Rabbit Monoclonal Antibody (SP4) to perform Western blotting Cyclin D1