siRNA / RNAi /miRNA transfection Human Lung Adenocarcenoma (A549/LTEP-a-2)

Get tips on using LC3B antibody to perform Autophagy assay cell type - CaCo-2

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized Saos-2

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized NTERA-2

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized EM-2

Get tips on using MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles to perform shRNA gene silencing Human - Islets of langerhans Negative control (scrambled) lentiviral particles

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using Anti-TIMP2 antibody [3A4] (ab1828) to perform Western blotting TIMP-2

Get tips on using Annexin V-FITC Apoptosis Kit to perform Necrosis MIA PaCa-2

Get tips on using Apoptosis and Necrosis Quantification Kit to perform Necrosis MIA PaCa-2