siRNA / miRNA gene silencing Human PC3 (human prostate cancer cell line)

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Human small intestinal organoids

Get tips on using Phospho-ULK1 (Ser757) Antibody to perform Autophagy assay cell type - Human primary MSCs

Get tips on using HTRA2 MISSION shRNA Lentiviral Transduction Particles HtrA serine peptidase 2 to perform shRNA gene silencing Mouse - FL83B HtrA2

Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Human melanocytes

Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - A2780

Get tips on using FxCycle™ PI/RNase Staining Solution to perform Cell cycle assay human - SW480

Get tips on using FxCycle™ PI/RNase Staining Solution to perform Cell cycle assay human - U266

Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - K562

Get tips on using ApopTag® Peroxidase In Situ Apoptosis Detection Kit to perform TUNEL assay cell type - FaDu human squamous cell carcinoma

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.