siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using SENSE mRNA-Seq Library Prep Kit V2 to perform RNA sequencing Human - Glioblastoma stem-like cells (GSCs)

Get tips on using GeneChip® HT 3' IVT PLUS Reagent Kit to perform RNA amplification & labeling Mammalian - RNA, Human Endometrial Stromal cells Biotin

Get tips on using Leukocyte Alkaline Phosphatase Kit to perform Acid phosphatase assay cell type - human periodontal ligament cells

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell culture media Human myogenic progenitor cells

Get tips on using Quant-iT™ RNA Assay Kit to perform RNA quantification Fuorimetric - human metastatic melanoma cells

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Mouse primary breast cancer ephitelial cells-Mammospheres

Get tips on using Absolutely RNA Nanoprep Kit to perform RNA isolation / purification Cells - primary rat dorsal root ganglion neurons

Get tips on using Aurum™ Total RNA Mini Kit to perform RNA isolation / purification Cells - primary mouse cortical neurons