rna-isolation-purification-cells-primary-mouse-dorsal-root-ganglion-neurons

Get tips on using FITC anti-mouse CD326 (Ep-CAM) Antibody to perform Flow cytometry Anti-bodies Mouse - CD326/EpCAM

Get tips on using ON-TARGETplus Mouse Stat5b (20851) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - FL83B Stat5b

Get tips on using ON-TARGETplus Mouse Cdh5 (12562) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - MS1 Cdh5

Get tips on using ON-TARGETplus Mouse Cd84 (12523) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - M210B4 Cd84

Get tips on using ON-TARGETplus Mouse Tead4 (21679) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - C2C12 Tead4

Get tips on using ON-TARGETplus Mouse Tead2 (21677) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - C2C12 Tead2

Get tips on using ON-TARGETplus Mouse Tead1 (21676) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - C2C12 Tead1

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.