siRNA / miRNA gene silencing Human hES cell line H1 (WA01)

Get tips on using Brilliant Violet 570™ anti-human CD27 Antibody to perform Flow cytometry Anti-bodies Human - CD27

Get tips on using Brilliant Violet 605™ anti-human CD69 Antibody to perform Flow cytometry Anti-bodies Human - CD69

Get tips on using PE/Dazzle™ 594 anti-human CD69 Antibody to perform Flow cytometry Anti-bodies Human - CD69

Get tips on using PerCP/Cyanine5.5 anti-human CD127 (IL-7Rα) Antibody to perform Flow cytometry Anti-bodies Human - CD127

Get tips on using Alexa Fluor® 647 Mouse Anti-Human CD24 to perform Flow cytometry Anti-bodies Human - CD24

Get tips on using Human Serpin E1/PAI-1 Quantikine ELISA Kit to perform ELISA Human - Serpin E1/PAI-1

Get tips on using Rabbit Anti-Human CHK2 (NT) Affinity Purified pAb to perform Immunohistochemistry chk2 - Rabbit IgG Human -NA-

Get tips on using CD326 (EpCAM) Antibody, anti-human, PE, REAfinity™ to perform Flow cytometry Anti-bodies Human - CD326/EpCAM

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.