siRNA / miRNA gene silencing Mouse mESC

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Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Primary cells Rat pulmonary artery smooth muscle cell (pPASMC)

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Primary cells Rat aortic smooth muscle cells (rASMC)

Get tips on using Senescence β-Galactosidase Staining Kit - Cell Signaling to perform Reporter gene assay β-galactosidase substrates - MCF-7 human breast cancer

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Get tips on using Senescence β-Galactosidase Staining Kit - Cell Signaling to perform Reporter gene assay β-galactosidase substrates - NHEK normal human epidermal keratinocytes

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Get tips on using Senescence β-Galactosidase Staining Kit - Cell Signaling to perform Reporter gene assay β-galactosidase substrates - HEK293 human embryonic kidney cells

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Get tips on using psiCHECK-2vector to perform Reporter gene assay luciferase - HEK 293 human embryonic kidney cells

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Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Gene specific profiling - MRC-5 BARX1

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Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Gene specific profiling - SiHa miR-375

Products Qiagen EpiTect Bisulfite Kit

Get tips on using EpiTect MSP Kit to perform DNA methylation profiling Gene specific profiling - Ca Ski MEG3

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Get tips on using EpiScope MSP Kit to perform DNA methylation profiling Gene specific profiling - HEK 293T NELL2

Products Takara Bio Inc EpiScope MSP Kit

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