Protein Expression Prokaryotic cells A. cellulolyticus

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I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

Discussions DNA insert using CRISPR

Get tips on using 2x Laemmli Sample Buffer to perform Protein isolation Bacteria - Vibrio alginolyticus

Products Bio-Rad Laboratories 2x Laemmli Sample Buffer

Get tips on using Strep-Tactin Superflow Plus (10 ml) to perform Protein tag Purification of Strep-tagged proteins

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Get tips on using Ni-NTA Fast Start Kit (6) to perform Protein tag Purification of His-tagged proteins

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Get tips on using Ni-NTA Magnetic Agarose Beads (6 x 1 ml) to perform Protein tag Purification of His-tagged proteins

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Get tips on using Laemmli Lysis-buffer to perform Protein isolation Bacteria - Clostridium difficile

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Get tips on using Laemmli Lysis-buffer to perform Protein isolation Bacteria - Bordetella pertussis

Products Sigma-Aldrich Laemmli Lysis-buffer

Get tips on using 2-D Quant Kit to perform Protein quantification Colorimetric method

Products Sigma-Aldrich 2-D Quant Kit

Get tips on using CelLytic™ B Cell Lysis Reagent to perform Protein isolation Bacteria - Fremyella diplosiphon

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Get tips on using CelLytic™ B Cell Lysis Reagent to perform Protein isolation Bacteria - Synechococcus elongatus

Products Sigma-Aldrich CelLytic™ B Cell Lysis Reagent

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