rna-isolation-purification-cells-primary-human-carotid-artery-endothelial-cells

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siRNA / miRNA gene silencing Human - H1299 Rab Coupling Protein (RCP) Experiment

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

RNA siRNA / miRNA gene silencing Human H1299 Rab Coupling Protein (RCP)

Senescence Cells Histochemical Staining Kit Product

Get tips on using Senescence Cells Histochemical Staining Kit to perform Reporter gene assay β-galactosidase substrates - mouse embryonic fibroblasts

Products Sigma-Aldrich Senescence Cells Histochemical Staining Kit

DMEM/F-12 Product

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Mouse primary breast cancer ephitelial cells-Mammospheres

Products Thermo Fisher Scientific DMEM/F-12

RNeasy Mini Kit Product

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat Submandibular glands