siRNA / miRNA gene silencing Human HeLa EPAS-1

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using GeneArt™ Site-Directed Mutagenesis PLUS System to perform Site Directed Mutagenesis (SDM) Human - Insertion SKOV3 miR-134

Get tips on using 1kb DNA Step Ladder to perform DNA Ladder 1 kb

Get tips on using BenchTop 1kb DNA Ladder to perform DNA Ladder 1 kb

Get tips on using GeneArt™ Site-Directed Mutagenesis System to perform Site Directed Mutagenesis (SDM) Mouse - 3T3-L1 S6 kinase 1

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - THP-1

Get tips on using Trichloroacetic acid to perform Protein isolation Mammalian cells - THP-1

Get tips on using Atg3 Antibody to perform Autophagy assay cell type - THP 1

Get tips on using LC3B Antibody to perform Autophagy assay cell type - THP 1

Get tips on using LC3B Antibody to perform Autophagy assay cell type - THP 1