siRNA / miRNA gene silencing Human MCF-7

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion TLN2

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion SLX4

Get tips on using phREX1-Luc to perform CRISPR Human - Activation REX1

Get tips on using SOX9 Polyclonal Antibody to perform Immunohistochemistry Human - SOX9

Get tips on using Dicer Antibody (CL0378) to perform Immunohistochemistry Human - Dicer1

Get tips on using MSH2 Polyclonal antibody to perform Immunohistochemistry Human - MSH2

Get tips on using CRISP3 Polyclonal antibody to perform Immunohistochemistry Human - CRISP3

Get tips on using Villin Monoclonal antibody to perform Immunohistochemistry Human - Villin

Get tips on using EZ-ChIP™ to perform ChIP Human - HUVEC

Stem cells have the unique ability to self-renew or differentiate themselves into various cell types in response to appropriate signals. These cells are especially important for tissue repair, regeneration, replacement, or in the case of hematopoietic stem cells (HSCs) to differentiate into various myeloid populations. Appropriate signals refer to the growth factor supplements or cytokines that mediate differentiation of various stem cells into the required differentiated form. For instance, HSCs can be differentiated into dendritic cells (with IL-4 and GM-CSF), macrophages (with m-CSF) and MDSCs (with IL-6 and GM-CSF). Human pluripotent stem cells (hPSCs) and induced pluripotent stem cells (iPSCs) can be first cultured in neural differentiation media (GSK3𝛃-i, TGF𝛃-i, AMPK-i, hLIF) to form neural rosettes, which can be differentiated into neural or glial progenitors (finally differentiated into oligodendrocytes). Neural progenitors can be finally differentiated into glutaminergic (dibytyryl cAMP, ascorbic acid) and dopaminergic (SHH, FGF-8, BDNF, GDNF, TGF-𝛃3) neurons. Thus, it is important to first identify the self-renewing cell line: its source and its final differentiation state, followed by the supplements and cytokines required for the differentiation, and final use. Timelines are another thing that is considered. For instance, it takes 7-10 days to form neural rosettes from iPSCs and 3 days to differentiate neural progenitors to neurons. Finally, the stability for stem cell culture media varies. It is advised to make fresh media every time when differentiating HSCs to myeloid populations, whereas neural differentiation media may remain stable for two weeks when stored in dark between 2-8C.