Protein expression and purification

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - Human lung fibroblasts

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - Mouse Epididymal fat

Get tips on using RIPA Buffer (10X) to perform Protein isolation Mammalian cells - Rat_Renal tissue

Get tips on using TRI Reagent® to perform Protein isolation Mammalian cells - Mouse_Brown fat

Get tips on using CelLytic™ M to perform Protein isolation Mammalian cells - Caco-2

Get tips on using CelLytic™ M to perform Protein isolation Mammalian cells - SH-SY5Y

Get tips on using CelLytic™ M to perform Protein isolation Mammalian cells - BHK-21

Get tips on using 2x Laemmli Sample Buffer to perform Protein isolation Bacteria - Vibrio alginolyticus

Get tips on using RIPA Lysis Buffer, 10X to perform Protein isolation Mammalian cells - HaCaT