siRNA / RNAi /miRNA transfection Human Lung Adenocarcenoma (A549/LTEP-a-2)

Get tips on using LC3A/B (D3U4C) XP® Rabbit mAb #12741 to perform Autophagy assay cell type - CaCo-2

Get tips on using Quick Start™ Bradford Protein Assay Kit 1 to perform Protein quantification Mammalian cells - BV-2

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - Colorectal aenocarenoma (SW48) - paraffin embeded

Get tips on using Subcellular Protein Fractionation Kit for Cultured Cells to perform Protein isolation Mammalian cells - Caco-2

I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

Get tips on using pSpCas9(BB)-2A-Puro (PX459) V2.0 to perform CRISPR Mouse - Deletion 3T3-L1 fmnl 2/3

Get tips on using QuantiFluor® dsDNA System to perform DNA quantification Human - Colorectal aenocarenoma (SW48) - paraffin embeded

Get tips on using FITC Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - Sao2-2, MG-62

Get tips on using Vybrant™ MTT Cell Proliferation Assay Kit to perform Apoptosis assay cell type - Sao2-2, MG-62

Get tips on using anti-p62 / SQSTM1 (C-terminus) guinea pig polyclonal, serum to perform Autophagy assay cell type - CaCo-2