Site Directed Mutagenesis (SDM) Human Deletion K562

Get tips on using VENTANA anti-MLH1 (M1) Mouse Monoclonal Primary Antibody to perform Immunohistochemistry Human - MLH1

Get tips on using Androgen Receptor (D6F11) XP® Rabbit mAb #5153 to perform Immunohistochemistry Human - AR

Get tips on using EZ‐ChIP™ Assay Kit (Cat#17–371) to perform ChIP Human - HeLa

Get tips on using SuperFect Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human aortic smooth muscle cells (HOSMC)

Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - BEAS-2B human bronchial epithelial cell line

Get tips on using GeneJET RNA Purification Kit to perform RNA isolation / purification Cells - primary human renal artery smooth muscle cells

Get tips on using CellROX™ Deep Red Reagent, for oxidative stress detection to perform ROS assay cell type - human umbelical vein endothelial cells (HUVEC)

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - primary human aortic smooth muscle cells

Get tips on using Gibco™ DMEM, high glucose, GlutaMAX™ Supplement to perform Stem cell culture media Human Dental pulp stem cells (hDPSC)

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.