Site Directed Mutagenesis (SDM) Human Point mutation THP-1

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Human gastric organoids

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Human endometrium organoids

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Human liver organoids

Get tips on using Silencer® Select_HDAC8 siRNA to perform siRNA / miRNA gene silencing Human - UCC HDAC8

Get tips on using Silencer® Select_SPRY2 siRNA to perform siRNA / miRNA gene silencing Human - RMS SPRY2

Get tips on using Silencer® Select_ MYD88 to perform siRNA / miRNA gene silencing Human - A375 MYD88

Get tips on using Silencer® Select_ TRIF to perform siRNA / miRNA gene silencing Human - A375 TRIF

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Seminal vesicles

Get tips on using CC10 Antibody (E-11): sc-365992 to perform Immunohistochemistry Human - SCGB1A1 /CC10