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Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human mononuclear cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human endothelial cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized HSG cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized MTD-1A

Get tips on using Wizard® Plus SV Minipreps DNA Purification System Technical Bulletin to perform Plasmid Isolation Streptomyces spp

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using De Man, Rogosa and Sharpe (MRS) Broth to perform Bacterial cell culture media Lactobacillus plantarum