Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?
When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel
Get tips on using Anti-trimethyl-Histone H3 (Lys9) Antibody to perform ChIP Anti-bodies H3K9me3
Get tips on using Anti-trimethyl-Histone H3 (Lys27) Antibody to perform ChIP Anti-bodies H3K27me3
Get tips on using Anti-trimethyl-Histone H3 (Lys9) Antibody to perform ChIP H3K9me3 - Sheep Rat -NA-
Get tips on using Anti-trimethyl-Histone H3 (Lys4) Antibody to perform ChIP H3K4Me3 - Sheep Rat -NA-
Get tips on using Anti-trimethyl-Histone H3 (Lys27) Antibody to perform ChIP H3K27me3 - Sheep Rat YFP Tag
Get tips on using TiterTACS™ Colorimetric Apoptosis Detection Kit to perform TUNEL assay cell type - Mouse endothelial cells
When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
Get tips on using Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315, rabbit monoclonal to perform ChIP Anti-bodies H3K4me3
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment