ChIP H3K4Me3 Rabbit

Get tips on using Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (ab7766) to perform ChIP Anti-bodies H3K4me2

Get tips on using Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (ab8895 to perform ChIP Anti-bodies H3K4me1

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

Get tips on using Rabbit anti-calretinin to perform Immunohistochemistry Mouse - Calb2

Get tips on using SOLiD™ ChIP-Seq Kit, with ChIP magnet to perform ChIP Human - SH-SY5Y

Get tips on using Anti-CCSP - Rabbit to perform Flow cytometry Anti-bodies Mouse - CCSP

Get tips on using LC3B Rabbit Antibody to perform Autophagy assay cell type - HEK 293

Get tips on using LC3B Rabbit Antibody to perform Autophagy assay cell type - SH-SY5Y

Get tips on using FOXP3 Rabbit Polyclonal Antibody to perform Western blotting FOXP3

Get tips on using PDGF Receptor β (28E1) Rabbit mAb to perform Immunohistochemistry PDGFβR - Rabbit Mouse -NA-