ChIP H3K9me3 Rabbit

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Get tips on using SOLiD™ ChIP-Seq Kit, with ChIP magnet to perform ChIP Human - SH-SY5Y

Products Thermo Fisher Scientific SOLiD™ ChIP-Seq Kit, with ChIP magnet

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

Proteins ChIP Anti-bodies H3K4me2

Get tips on using Anti-CCSP - Rabbit to perform Flow cytometry Anti-bodies Mouse - CCSP

Products Seven Hills Bioreagents Anti-CCSP - Rabbit

Get tips on using LC3B Rabbit Antibody to perform Autophagy assay cell type - HEK 293

Products Cell Signaling Technology LC3B Rabbit Antibody

Get tips on using LC3B Rabbit Antibody to perform Autophagy assay cell type - SH-SY5Y

Products Cell Signaling Technology LC3B Rabbit Antibody

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.

Proteins ChIP Anti-bodies H3K4me1

Get tips on using FOXP3 Rabbit Polyclonal Antibody to perform Western blotting FOXP3

Products OriGene FOXP3 Rabbit Polyclonal Antibody

Get tips on using PDGF Receptor β (28E1) Rabbit mAb to perform Immunohistochemistry PDGFβR - Rabbit Mouse -NA-

Products Cell Signaling Technology PDGF Receptor β (28E1) Rabbit mAb

Get tips on using LAMP2 (D5C2P) Rabbit mAb to perform Autophagy assay cell type - GH3

Products Cell Signaling Technology LAMP2 (D5C2P) Rabbit mAb

Get tips on using Atg7 (D12B11) Rabbit mAb to perform Autophagy assay cell type - HaCaT

Products Cell Signaling Technology Atg7 (D12B11) Rabbit mAb

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