Get tips on using Histone H3K27ac antibody (pAb) to perform ChIP Anti-bodies H3K27ac
Get tips on using Histone H3K4me3 antibody (mAb) to perform ChIP Anti-bodies H3K4me3
Get tips on using MAGnify™ Chromatin Immunoprecipitation System to perform ChIP Rat - Heart
Get tips on using Imprint® Chromatin Immunoprecipitation Kit to perform ChIP Rat - Brain
Get tips on using MAGnify™ Chromatin Immunoprecipitation System to perform ChIP Mouse - C2C12
Get tips on using Imprint® Chromatin Immunoprecipitation Kit to perform ChIP Mouse - MIN6
Get tips on using MAGnify™ Chromatin Immunoprecipitation System to perform ChIP Mouse - RAW264.7
Get tips on using Imprint® Chromatin Immunoprecipitation Kit to perform ChIP Mouse - RAW264.7
Get tips on using MAGnify™ Chromatin Immunoprecipitation System to perform ChIP Mouse - NIH3T3
Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.
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