PCR Methylation specific PCR

Get tips on using QuantiTect Virus +ROX Vial Kit (1000) to perform PCR Quantitative real-time PCR - Viral

Get tips on using FastLane Cell SYBR Green Kit (200) to perform PCR Quantitative real-time PCR - Viral

Get tips on using Kapa Biosystems HIFI HOTSTART READY MIX to perform PCR Hot start PCR - Bacterial DNA

Get tips on using TEMPase Hot Start Master Mix BLUE to perform PCR Hot start PCR - Bacterial DNA

Get tips on using Phusion Hot Start II DNA Polymerase to perform PCR Hot start PCR - Bacterial DNA

Get tips on using Fast SYBR™ Green Master Mix to perform PCR Quantitative real-time PCR - Bacterial DNA

Get tips on using Hot StarTaq Master Mix Kit (2500 U) to perform PCR Hot start PCR - Mammalian DNA

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using LightCycler® 480 SYBR Green I Master to perform PCR Quantitative real-time PCR - Mammalian DNA

Get tips on using Brilliant II SYBR Green qPCR Master Mix to perform PCR Quantitative real-time PCR - Mammalian DNA