Site Directed Mutagenesis (SDM) Rat Point mutation

Get tips on using Rat Cytochrome c(CYCS) ELISA kit to perform ELISA Rat - Cytochrome c

Get tips on using Rat Bone Morphogenetic Protein 2 ELISA to perform ELISA Rat - BMP-2

Get tips on using Anti-LC3 (Rat) pAb to perform Autophagy assay cell type - OE21

Get tips on using Rat ICAM1 ELISA Kit (CD54) (ab100763) to perform ELISA Rat - ICAM-1/CD54

Get tips on using Rat Endothelial Cell Growth Medium to perform Mammalian cell culture media RAOEC

Get tips on using Biotin Rat Anti-CD11b to perform Flow cytometry Anti-bodies Mouse - CD11b

Get tips on using Anti-LC3 (Rat) pAb to perform Autophagy assay cell type - MLO-Y4

Get tips on using Anti-LC3 (Rat) pAb to perform Autophagy assay cell type - MLO-Y4

Get tips on using Rat PAI1 ELISA Kit (SERPINE1) (ab198509) to perform ELISA Rat - Serpin E1/PAI-1

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.