RNA isolation / purification Cells Cancer cell lines Leukemia cancer cell lines

Acid phosphatase detection heavily relies on determining the concentration of tartrate-resistant acid phosphatase (TRAP) in the sample. Hence, sample preparation is very crucial and it should be done strictly as per kit manufacturer instructions to avoid any inconsistency and poor sensitivity

Get tips on using LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit to perform Live / Dead assay mammalian cells - mouse, T-cell

Get tips on using MojoSort™ Human Pan Monocyte Isolation Kit to perform Cell Isolation Monocyte

Get tips on using Slan (M-DC8)+ Monocyte Isolation Kit, human to perform Cell Isolation Monocyte

Get tips on using EasySep™ Direct Human Monocyte Isolation Kit to perform Cell Isolation Monocyte

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using Cultrex® BME Cell Invasion Assay to perform Cell migration / Invasion cell type - T47D

Get tips on using Cultrex® BME Cell Invasion Assay to perform Cell migration / Invasion cell type - HepG2

Get tips on using Cultrex® BME Cell Invasion Assay to perform Cell migration / Invasion cell type - A549

Get tips on using Cultrex® BME Cell Invasion Assay to perform Cell migration / Invasion cell type - HUVEC