Site Directed Mutagenesis (SDM) Rat Point mutation

Get tips on using CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS) to perform Cell cytotoxicity / Proliferation assay cell type - MG-63

DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.

Get tips on using SbfI-HF® to perform Restriction Enzymes SbfI / SdaI

Get tips on using Bsp1286I NEB#R0120 to perform Restriction Enzymes Bsp1286I / SduI

Get tips on using Human Syndecan-1 DuoSet ELISA to perform ELISA Human - SDC1

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Human - SMMC-7721

Isolating RNA from tissues and paraffin embeded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the intigrity of RNA

Isolating RNA from tissues and paraffin embeded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the intigrity of RNA