PCR Quantitative real-time PCR

Get tips on using REPLI-g Advanced DNA Single Cell Kit (96) to perform Whole Genome Amplification Mouse

Get tips on using KnockOut™ Serum Replacement - Multi-Species to perform Stem cell Differentiation media Human Limbal Epithelial cells

Get tips on using Gibco™KnockOut™ Serum Replacement to perform Stem cell Differentiation media hESCs differentiation into cortical neuroepithelium (NE)

Get tips on using pCcrtB to perform Protein Expression Prokaryotic cells - E. coli Kocuria gwangalliensis CrtB

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using pCX-puro-mouLH to perform Protein Expression Eukaryotic cells - CHO mouse LH

Get tips on using pCX-puro-mouFSH to perform Protein Expression Eukaryotic cells - CHO mouse FSH

Acid phosphatase detection heavily relies on determining the concentration of tartrate-resistant acid phosphatase (TRAP) in the sample. Hence, sample preparation is very crucial and it should be done strictly as per kit manufacturer instructions to avoid any inconsistency and poor sensitivity

Get tips on using pCW-LIC to perform Protein expression and purification Bacteria - Escherichia coli Fbxo7

Get tips on using pCEP4 Mammalian Expression Vector to perform Protein expression and purification Bacteria - Origami™ B (DE3) ABCA4