Get tips on using ExpressArt FFPE Clear RNAready to perform RNA isolation / purification Tissue - Rat Kidney
Get tips on using ExpressArt FFPE Clear RNAready to perform RNA isolation / purification Tissue - Human Kidney
Get tips on using ExpressArt FFPE Clear RNAready to perform RNA isolation / purification Tissue - rat kidney tissue
Get tips on using ExpressArt FFPE Clear RNAready to perform RNA isolation / purification Tissue - human kidney tissue
Get tips on using pcDNA™3.1 (+) Mammalian Expression Vector to perform siRNA / miRNA gene silencing Human - U251 cofilin-1 (CFL1)
Get tips on using Midnight RT PCR Expansion to perform PCR Conventional / Qualitative PCR - Viral DNA
Get tips on using pMIR-REPORT™ miRNA Expression Reporter Vector System to perform Reporter gene assay luciferase - HEK 293 human embryonic kidney cells
DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.
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