shRNA gene silencing Mouse Prostate cancer cell lines (DU145 and PC3)

Get tips on using EmbryoMax MEF Conditioned Media to perform Stem cell culture media Mouse trophoblast stem cells

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell culture media Mouse myoblasts cells

Get tips on using Anti-p62 (SQSTM1) (Human) pAb to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.