Get tips on using Pan Ras Monoclonal Antibody (Ras10) to perform Western blotting Ras
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
Get tips on using Donkey anti-rabbit IgG to perform Immunohistochemistry Anti-rabbit IgG - Donkey Rabbit Rhodamin red
Get tips on using RAC1 Antibody to perform Western blotting Rac1
Get tips on using RAC1 Polyclonal Antibody to perform Western blotting Rac1
Get tips on using High Range DNA Ladder to perform DNA Ladder Long Range
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