The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
Get tips on using Autophagy Assay Kit to perform Autophagy assay cell type - A549
Get tips on using Anti-Atg5 to perform Autophagy assay cell type - Mouse white adipose tissue
Get tips on using Aspergillus antibody | WF-AF-1 to perform Immunohistochemistry Mouse - Aspergillus
Get tips on using ABC1 Antibody (AB.H10): sc-58219 to perform Western blotting ABCA1
Get tips on using APG5 Antibody (C-1) to perform Autophagy assay cell type - A549
Get tips on using Anti-Vimentin antibody (ab137321) to perform Flow cytometry Anti-bodies Human - Vimentin
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment