siRNA / miRNA gene silencing Human U937 MK2 (MAPK Kinase 2)

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Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Immortalized cell lines 293T

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Human - Deletion RNase L

Products Addgene pSpCas9(BB)-2A-GFP (PX458)

Get tips on using E-Cadherin (24E10) Rabbit mAb #3195 to perform Immunohistochemistry Human - E-Cadherin

Products Cell Signaling Technology E-Cadherin (24E10) Rabbit mAb #3195

Get tips on using FGF-10 Antibody (3C7): sc-293208 to perform Immunohistochemistry Human - FGF-10

Products Santa Cruz Biotechnology FGF-10 Antibody (3C7): sc-293208

Get tips on using Mucin 5AC Antibody (45M1): sc-21701 to perform Immunohistochemistry Human - Muc-5AC

Products Santa Cruz Biotechnology Mucin 5AC Antibody (45M1): sc-21701

Get tips on using pSpCas9(BB)-2A-Puro (PX459) V2.0 to perform CRISPR Human - Activation ERαY537S

Products Addgene pSpCas9(BB)-2A-Puro (PX459) V2.0

Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Gene specific profiling - TCP-1, BCPAP & nthy-ori 3-1 (thyroid tumor cells) METTL7A

Products Qiagen EpiTect Bisulfite Kit

Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Gene specific profiling - TCP-1, BCPAP & nthy-ori 3-1 (thyroid tumor cells) BCPAP

Products Qiagen EpiTect Bisulfite Kit

Get tips on using KAPA Stranded mRNA-Seq Kit to perform RNA sequencing Human - SKBR-3

Products Roche Lifesciences KAPA Stranded mRNA-Seq Kit

Get tips on using KAPA Stranded mRNA-Seq Kit to perform RNA sequencing Human - MCF-7

Products Roche Lifesciences KAPA Stranded mRNA-Seq Kit

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